Excellent Flow Cytometry Antibody For CD134 (OX40)

December 12, 2016

BlueskyReddit
Microbiology and Immunology
UIC
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

Anti-Mouse CD134 (OX40) PE

Catalog Number:

12-1341-81

I used to this antibody to determine OX40 expression in CD4+ T-cells from mouse spleen, thymus and lymph nodes.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse T-cells

Primary Incubation

1:200 for 3h at 4 degree

Blocking Agent

0.5% BSA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

I always get a nice distinct OX40+ population of T-cells which is of presentable quality.

DOI or PMID #

NA

Additional Notes

Works well before and after permeabilization

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Summary

The Good

Ease of optimization and reproducible results

The Bad

Little expensive

The Bottom Line

Worth buying it

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