Time Course Effect Of Chemoattractant On Galpha-Q Expression

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Santa Cruz Biotechnology

Product Name:

Galpha-q

Catalog Number:

Sc-393

G-protein alpha-q belongs to trimeric G-protein alpha-q/ G-protein beta/gamma family. G proteins are activated within the cell by G protein-coupled receptors (GPCRs) that span the cell membrane. Here we checked the time course effect of chemoattractant on mouse macrophages.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse cell lysate (specifically macrophages)

Primary Incubation

5% milk prepared in 1x TBST in 1:1000 dilution of primary anbtobody raised in mouse

Blocking Agent

5% milk prepared in 1x TBST

Secondary Incubation

5% milk prepared in 1x TBST in 1:4000 dilution

Tertiary Incubation

None

Detection

ECL solution

Results Summary

We found time dependent increase of Galpha-q by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Very good for western blot

The Bad

None

The Bottom Line

This antibody is very efficient for western blot and the results are accurate. Highly recommended.

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