Expression of MCM3 in Human Aortic Smooth Muscle Cells

Pathology
University of Tennessee Health Science Centre
Postdoctoral Fellow

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Company:

Abcam

Product Name:

Anti-MCM3 Antibody

Catalog Number:

ab4460

MCM3 is a component of hexameric protein complex (MCM2-7), which are involved in the initiation of eukaryotic genome replication. The aim of the present experiment is to detect the expression of MCM3 protein in human aortic smooth muscle cells by western blot. The antibody (ab4460) used here gave clean specific band at right size.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human Aortic Smooth Muscle (HASM) Cells - Whole Cell Lysate

Primary Incubation

1:2000

Blocking Agent

10% Milk for 1 Hour

Secondary Incubation

1:4000

Tertiary Incubation

NA

Detection

ECL

Results Summary

Antibody detected the MCM3 protein in HASM cells at around 105 kDa.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

Image Gallery

Summary

The Good

Identifies clear band at right size

The Bad

Nothing

The Bottom Line

Antibody detected MCM3 at around 105 kDa

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