Excellent Flow Cytometry Antibody For Phopho S6 Detection

November 23, 2016

BlueskyReddit
Microbiology and Immunology
UIC
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

Anti-Human/Mouse phospho-S6 Ribosomal (S235/S236) PE

Catalog Number:

12-9007-41

I used to this antibody to study S6 phosphorylation in T-cells and antibody works fantastically.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse T-cells

Primary Incubation

1:100 for 3h at 4 degree

Blocking Agent

0.5% BSA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

I got distinct S6 phosphorylated and non-phosphorylated T-cell population with/without stimulation cocktail. Results were of presentable quality.

DOI or PMID #

NA

Additional Notes

I used Foxp3 transcription Fix/Permeabilation Kit from eBioscience for fixation and permeabilization

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Summary

The Good

Ease of optimization and reproducible results

The Bad

Little expensive

The Bottom Line

Worth buying it

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