anti-Mouse IgG Fc Secondary Antibody

November 30, 2016

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen by Thermo Fisher Scientific

Product Name:

Goat anti-Mouse IgG Fc Secondary Antibody, HRP

Catalog Number:

31437

To study the time course effect of chemoattractant on ABCA1 expression in mouse macrophages. This was used as secondary antibody to detect the signal in western blot for the primary antibodies raised in mouse.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse cell lysate

Primary Incubation

5% milk prepared in 1x TBST in 1:1000 dilution of primary anbtobody raised in mouse

Blocking Agent

5% milk prepared in 1x TBST

Secondary Incubation

5% milk prepared in 1x TBST in 1:4000 dilution

Tertiary Incubation

None

Detection

ECL

Results Summary

This secondary antibody is easy to prepare and efficient in detecting bands in western blot.

DOI or PMID #

N/A

Additional Notes

None

Image Gallery

Summary

The Good

Detect the band very strongly

The Bad

1hr incubation is enough to detect the band. Long incubation will leads to non specific binding

The Bottom Line

It is very helpful in detecting the signal for proteins raised in mouse. Highly recommended.

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