Abcam
Anti-beta Actin antibody [AC-15]
ab6276
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Browse Similar Products: Anti-beta Actin Western Blot Antibodies
Our projects require the testing of various inflammatory stimuli or agonists on peritoneal macrophages from mice that are either drug-treated or untreated. To confirm or test our hypotheses on what targets may have been modulated upon inflammatory stimuli and drug treatment, we utilize best practice western blot techniques to analyze and measure the protein/target of interest in our experiments.
Western Blot
Mouse peritoneal macrophages
1/10000 of Ab in 5% milk in TBST, overnight, 4 deg C
Non-fat dry milk proteins in TBS
1/10000 of HRP-Ab in 5% milk in TBST, 1 hour, room temperature
None
X-Ray development using ECL and photofilm
The peritoneal macrophages were sorted using flow cytometry and the drug's inherent fluorescent signature. See - https://www.ncbi.nlm.nih.gov/pubmed/26109497. Post-sorting, the cells were plated for 24-48 hours before the agonists or inflammatory stimuli were applied. 8-24 hours post stimulus, cells were collected and lysed, measure for protein concentration and best practice western blot techniques were used as discussed above. As seen in the figure, the repeatable band production of b-actin in three different mice samples was easily verified suggesting equivalent protein loading in our gel.
10.1002/cyto.a.22706
Easy to use and consistent results
A very effective loading control for western blot analysis of mouse peritoneal macrophages.