Antibody Review For CD11c

November 23, 2016

Cell and Molecular Biology
Northwestern University
Research Associate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Molecular Probes, Invitrogen

Product Name:

CD11c Antibody, Integrin alpha X FITC conjugate (3.9)

Catalog Number:

A25988

The overall goal of my research is to study transmission of SIV/HIV. I specifically wanted to test what is the prevalence of CD11c positive cells across different tissues. This antibody was chosen as it was previously reported to work well in Rhesus Macaque for the purpose of fluorescent microscopy and it was already available in our laboratory.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Rhesus macaque tissue

Primary Incubation

One hour at room temperature

Blocking Agent

PIPES/Formaldehyde

Secondary Incubation

None

Tertiary Incubation

None

Detection

Microscopy

Results Summary

Many CD11c positive cells were identified across different tissues. CD11c positive cells come in many shapes from round and spherical to cells having many branched projections.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Easy one our incubation

The Bad

FITC conjugated antibodies are not optimal for imaging tissues due to high background

The Bottom Line

I would recommend this antibody however in future I would use different color secondary to combat the background of the tissue due to autofluorescence.

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