BD Biosciences
APC Mouse Anti-Human CD209
551545
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Browse Similar Products: CD209 Flow Cytometry Antibodies
The goal of my experiment was to assess the effect of serum from two different sources on the conversion of peripheral blood CD14 monocytes into dendritic cells using GM-CSF and IL-4. This product was selected because of the fluorochrome conjugate APC, as it is very bright and does not spillover into other channels.
Flow Cytometry
Human monocyte-derived dendritic cells (moDCs)
1:200, 20 mins, 4 degree C.
Trustain Human FcX
None
Red laser (633 nm)
Significant difference was seen in the ability of the sera from two sources to convert CD14+ monocytes into CD1a+CD209+ moDCs.
N/A
The data sheet that comes with this reagent mentions to use 20 microlitres per test. However, in my hands, it works well enough at a dilution of 1:200.
Works well at high dilution and thus cost-effective.
High upfront cost
Great antibody overall. Definitely titrate and use sparingly.