Red AP Substrate Kit: Works Well and Super Easy to Use

November 28, 2016

UCSF
Urology
Grad Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Vector

Product Name:

VECTOR Red Alkaline Phosphatase (Red AP) Substrate Kit

Catalog Number:

SK-5100

The kit is very easy to use and the staining looked great on embryonic stem cells.

Experimental Design and Results Summary

Application

Alkaline Phosphatase

Starting Material

Embryonic stem cells

Protocol Overview

Fix cells in PFA, wash with PBS, put on Vector Red AP substrate for 30 minutes, then image.

Tips

Followed the protocol and everything worked the first time

Results Summary

Images looked great.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy to use and works

The Bad

None

The Bottom Line

I will purchase this kit again and recommend it to anyone for Alkaline Phosphatase staining.

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