Expression of ORC3 in Human Aortic Smooth Muscle Cells

Pathology
University of Tennessee Health Science Centre
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology

Product Name:

Anti-ORC3 Antibody

Catalog Number:

sc-374231

The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. Here we checked the expression of ORC3 protein in Human Aortic Smooth Muscle Cells by Western Blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human Aortic Smooth Muscle (HASM) Cells - Whole Cell Lysate

Primary Incubation

1:200

Blocking Agent

10% Milk for 1 Hour

Secondary Incubation

1:4000

Tertiary Incubation

NA

Detection

ECL

Results Summary

Antibody detected clear band at right size (80 kDA)

DOI or PMID #

NA

Additional Notes

NA

Related Categories

Image Gallery

Summary

The Good

Easy to optimise and produce right band at right size

The Bad

Nothing

The Bottom Line

Antibody detected clear band at right size (80 kDA)

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