Expression of MCM2 in Human Aortic Smooth Muscle Cells

Pathology
University of Tennessee Health Science Centre
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-MCM2 Antibody

Catalog Number:

ab4461

MCM2 - Mini-chromosome maintenance component 2, which is a component of MCM complex, which is essential for pre-replication complex (pre-RC) and may be involved in the formation of replication forks. Here we detected the expression of MCM2 protein in Human Aortic Smooth Muscle Cells by Western Blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human Aortic Smooth Muscle (HASM) Cells - Whole Cell Lysate

Primary Incubation

1:2000

Blocking Agent

10% Milk for 1 Hour

Secondary Incubation

1:4000

Tertiary Incubation

NA

Detection

ECL

Results Summary

Antibody detected the clear MCM2 band around 100 kDA in the whole cell lysate of HASMCs.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

Image Gallery

Summary

The Good

Easy to optimise and produce right band at right size

The Bad

Nothing

The Bottom Line

Antibody detected the clear MCM2 band around 100 kDA

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