BD Biosciences
BUV395 Rat Anti-Mouse CD11b
563553
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Browse Similar Products: CD11b Flow Cytometry Antibodies
This antibody is used as part of a gating strategy to detect monocyte/neutrophils in murine blood and Kupffer cells/monocytes from murine livers. Results using this antibody on murine blood have been published (Elife. 2016 Jul 27;5. pii: e13024. doi:10.7554/eLife.13024. PubMed PMID: 27462873; PubMed Central PMCID: PMC4963201.)
Flow Cytometry
Murine blood/liver
1:800, 4C, 10-20 million cells per ml, 20 to 30 min stain
Anti CD16/CD32 at 1:1000
NA
Flow cytometry, 355 nm laser, 389/38-25 bandpass filter (Semrock).
BUV395 doesn't separate as well as other brighter stains, but for highly expressed marks, it works great. Further, there is almost no need for compensation, which can be useful while looking at many myeloid marks in parallel.
27462873
Minimal compensation, great clone
Population separation with BUV395 isn't as good as using brighter dyes.
I have ordered this product at least 5 times now.