Anti-MRE11 antibody from Bethyl

November 14, 2016

Pharmacology
Boston University
Senior Post-doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Bethyl Laboratories

Product Name:

Rabbit anti-Mre11 Antibody

Catalog Number:

A300-181A

MRE11 is a component of MRN complex required for DNA recombination, DNA double-strand break repair, and telomere integrity. It associates with NBS1 and Rad50 to form MRN complex. Using this antibody for Western blotting help to test whether MRE11 still interact with its binding partners under different situations.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human HeLa cell line

Primary Incubation

1:1000 in TBS-T at Room Temperature for 2hr

Blocking Agent

5% milk in TBS-T

Secondary Incubation

Anti-Rabbit-HRP at 1:5000 in TBS-T

Tertiary Incubation

N/A

Detection

Bio-Rad ECL

Results Summary

This antibody detects a doublet of MRE11 that migrate between 75 and 100 kDa markers. The antibody is specific and the background is quite clean.

DOI/PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Specific and clean background

The Bad

The sensitivity is a little weaker, but can be overcame by using more antibody

The Bottom Line

Overall this is a good MRE11 antibody that can be used to detect the protein level. Whether this antibody is also good for other applications such as immunofluorescence microscope needs to be tested.

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