Mouse Anti Sheep CD44 (Purified) From Bio-Rad

Wisconsin National Primate Research Center
University of Wisconsin-Madison
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BIO-RAD

Product Name:

Mouse anti Sheep CD44

Catalog Number:

MCA2219GA

We used this antibody to check the CD44 expression of sheep skin fibroblast and its derived iPSCs for flow. It gave very good separation of different population.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Sheep skin fibroblast

Primary Incubation

5ul antibody in 50ul cell suspension, incubate in 4 degree for 15 min

Blocking Agent

N/A

Secondary Incubation

Alexa Fluro 488 goat anti-mouse IgG was 1:1000 diluted and incubated in in 4 degree for 15 min

Tertiary Incubation

N/A

Detection

MACSQuant

Results Summary

This antibody worked excellently in FACS analysis of sheep fibroblast cells expressing CD44.

Additional Notes

None

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Summary

The Good

Works very well.

The Bad

A little bit expensive.

The Bottom Line

This is very good antibody to check the expression of CD44 on sheep skin fibroblast.

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