BD Biosciences
BD OptiBuild™ BV786 Rat Anti-Mouse CD31
740870
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I use this antibody in an 8 color panel for sorting/identification of endothelial cells from murine livers on a MoFlo Astrios equipped with a 405 nm laser and detected by a 795/70 filter. It spills some into the channel for PE-Cy7 (561:795/70) and APC-Cy7 (642:795/70) but is easily corrected by compensation.
Flow Cytometry
Murine liver
1:600, 4C, 10-30 million cells per ml, 20 to 30 min stain
Anti CD16/CD32 at 1:500
NA
Flow cytometry, 405 nm laser, 795/70 bandpass filter.
Nice separation on non-parenchymal cells from in situ digested livers. Doesn't work well if tissue is digested with Pronase E but works well when digested with Liberase thermolysin medium.
None
Bright and easy to compensate.
Shipping takes awhile as it is Opti-Build and made to order.
I like the results and have ordered it three times.