KingFisher System - Perfect Solution For Customizable Automated Experiment Design

November 04, 2016

BlueskyReddit
UChicago
Chem
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Company:

Thermo Fisher

Product Name:

KingFisher™ Duo Prime Purification System

Catalog Number:

5400110

The aim of my research is the study of RNA modifications, which requires a lot of parallel RNA purification and IP experiments. KingFisher enables me to process up to 12 samples at a time with automated washing and beads separation steps, which saves a lot of time and efforts.

Experimental Design and Results Summary

Application

RNA IP using magnetic beads

Starting Material

Sonicated RNA

Protocol Overview

Use the supplied Bindit software to design each processing step (see images), load samples and reagents accordingly, hit run and return after the estimated time to collect products.

Tips

make sure to work in a clean area and put the deep well plate on ice

Results Summary

The IP steps are processed in parallel with minimal handling time and discrepancy between samples. The recovery efficiency is very high.

Features Summary

Customizable automation, parallel sample processing

Additional Notes

None

Image Gallery

Summary

The Good

The processing steps are fully customizable and automated, the machine is robust and requires little attention

The Bad

The machine cannot move liquid from one well to another, limiting the application

The Bottom Line

Cost-efficient automation solution for magnetic beads-based experiments.

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