Abcam
Anti-VEGF Receptor 3 antibody
ab27278
Info: View Product View Product Specs
Browse Similar Products: VEGF Western Blot Antibodies
Aim of the study was to detect differential expression of angiogenesis related VRGF receptor 3 proteins in response to different treatments. Animals maintained at different experimental conditions were sacrificed following ethical norms. Rat tissues were harvested, proteins were extracted and an equal amount of proteins were resolved in SDS-PAGE and immunoblotted for VEGFR3. Abcam antibody was selected because of information provided in antibody flyers.
Western Blot
Rat tissue
Overnight at 4 0C (5% Skimmed milk in TBS with 0.1% Tween 20; dilution 1:1000)
5 % Skimmed milk in TBS with 0.1% Tween 20 (for 1hr at RT)
2hr at RT (5% skimmed milk in TBS with 0.1% Tween 20; dilution 1:4000)
None
ECL
Although, bands were not very clean, a significant difference in levels of VEGFR3 protein expression was observed in response to treatment.
Got specific band of VEGFR3
Got some non specific bands very near to target protein.
Average specificity of Abcam VEGFR3 antibody