Immunofluorescence Luciferase Antibody

Cancer Centre
Cold Spring Harbor Laboratory
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus

Product Name:

Luciferase

Catalog Number:

NB100-1677

The goal was to see if we had luciferase signal in our RapidCaP model's prostate. We selected this product because there are not many working antibodies.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse Prostate

Primary Incubation

10mM Citrate Buffer

Blocking Agent

10% natural goat serum in PBS

Secondary Incubation

dilated Luciferase ab in blocking buffer

Tertiary Incubation

2nd fluorescent-conjugated ab diluted in PBS

Detection

DAPI

Results Summary

Great, publishable results. Luciferase shown in our model.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

This is the only ab we got to work

The Bad

N/A

The Bottom Line

This Ab was the only one that we are able to publish with.

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