Maxiprep Plasmid Purification Kit

George Washington University
Cancer Center
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

ThermoFischer Scientific

Product Name:

PureLink® HiPure Plasmid Filter Maxiprep Kit

Catalog Number:

K210016

In the lab, we routinely purify large amounts of plasmids for lentiviral transfection of CD8 T cells to engineer their specificity against immunodominant HIV-1 peptides, and enhance their anti-HIV activity. As such, we use E coli and the maxiprep system to grow and purify the plasmids of interest.

Experimental Design and Results Summary

Application

Plasmid DNA purification

Starting Material

E. coli bacterial culture pellet

Protocol Overview

We follow the protocol provided in the kit step by step.

Tips

Just follow the protocol

Results Summary

We get a very good yield from the kit, usually 1-2 mg/ml of DNA.

Additional Notes

None

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Summary

The Good

Works very well

The Bad

None

The Bottom Line

I recommend this kit.

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