A Good Total RNA Purification Kit, Especially For Small RNAs

Boston University
Pharmacology
Senior Post-doc

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Quality of Results

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Company:

Thermo Scientific

Product Name:

mirVana miRNA Isolation Kit, without phenol

Catalog Number:

AM1561

This kit from ambion (Thermo Fisher) is a good option for purification of total RNA, especially the small RNAs. The kit provides a rapid procedure that allows researchers to isolate small RNAs efficiently. It is ideal for miRNA, snRNA and shRNA analysis and can be used for different cell types and tissues.

Experimental Design and Results Summary

Application

RNA analysis

Starting Material

A variety of cell types

Protocol Overview

The manual from the manufacturer is easy to understand and follow.

Tips

Inhibition of RNase activity and thorough disruption of cells/tissues

Results Summary

Good yield and clean purification with reproducible quality.

Additional Notes

N/A

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Summary

The Good

Quality and consistency

The Bad

N/A

The Bottom Line

Compared to other products, this one separates RNA size better and always gives good quality RNAs for RNA-seq.

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