Berthold Technologies
AutoLumat LB 953 Multi-Tube Luminometer
LB-953
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We generally use luciferase reporter HIV virus for our experiments and use luciferase levels as a mean to determine infection levels. This machine is extremely valuable because it can detect only a few molecules of the luciferase enzyme and can precisely determine differences in luciferase activity between the different samples. we could not get this level of precision by using a regular plate reader luminometer.
Luciferase quantification
Human cells infected with luciferase reporter HIV virus
Place the samples in the 5ml polystyrene round bottom tubes (volume depends on the luciferase assay you are using). Place a tube with the appropriate volume of assay substrate on the injector then prime the machine with the substrate. Set the parameters of the assay such as: injection volume, delay time, reading time. Start a new assay, the machine will count the number of tubes and ask you to save the workload. Save and press go. The machine does the rest.
Add a few empty tubes (one at the beginning and one at the end of the load for background measurement.
The machine gives you raw RLU values for each samples. The max RLU detected is just under 100 million. and the background values are usually at around 100RLU. This allows you to detect a very wide range of luciferase levels which is useful when you are comparing different treatment conditions.
Fully automated injection of up to 3 solutions.
None
Highly sensitive, easy to use
Only works with an old PC computer.
Great machine to use if you rely on luciferase quantification for your experiments.
Product Name: AutoLumat Multi-Tube Luminometer LB 953Supplier Page