Highly Sensitive Luciferase Machine

Albert Einstein College of Medicine
Microbiology & Immunology
PhD Candidate

Overall

Performance

Ease-of-Optimization

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Company:

Berthold Technologies

Product Name:

AutoLumat LB 953 Multi-Tube Luminometer

Catalog Number:

LB-953

We generally use luciferase reporter HIV virus for our experiments and use luciferase levels as a mean to determine infection levels. This machine is extremely valuable because it can detect only a few molecules of the luciferase enzyme and can precisely determine differences in luciferase activity between the different samples. we could not get this level of precision by using a regular plate reader luminometer.

Experimental Design and Results Summary

Tips

Add a few empty tubes (one at the beginning and one at the end of the load for background measurement.

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Summary

The Good

Highly sensitive, easy to use

The Bad

Only works with an old PC computer.

The Bottom Line

Great machine to use if you rely on luciferase quantification for your experiments.

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