Stemcell Technologies
EasySep™ Biotin Positive Selection Kit
18559
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We use this kit to purify cells that have been successfully transduced with a lentivirus expressing a truncated mouse reporter protein. It can be used for any kind of cell selection when using the appropriate biotynilated antibody.
Cell separation
Transduced human CD8+ T cells
Cells are resuspended at 10^8 cells/ml. Add biotynilated antibody at optimal concentrations (I use 1:100), incubate for 15min at room temperature (RT). Without washing add biotin selection coctain at 1:10, incubate 15min at RT. Mix magnetic particles and add 50ul/ml., incubate 10min at RT. Bring volume up to 5ml, place in magnet for 5min. Wash 3x with 5ml of buffer. Resuspend positive cells as desired.
Follow the protocol.
We get very high purity of cells expressing our reporter protein (>90%). Cells are viable and not affected by the purification.
None
High purity, easy protocol
Good kit to use for purification of cells based on expression of surface markers/reporters.
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