Human CD4 Antibody

Cancer Center
George Washington University
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PerCP/Cy5.5 anti-human CD4 Antibody

Catalog Number:

317428

Our laboratory is investigating different ways to enhance immune responses against HIV. As such, we are studying novel approaches to engineer or stimulate effector cells such as CD8 T cells, or NK cells for increased clearance of HIV-infected cells. CD4+ cells are the primary target of HIV, and we routinely purify human CD4 T cells from PBMC to infect and set up in vitro killing assays. Good, specific antibodies are needed to confirm good purification of cells, as well as distinguish effector from CD4+ target cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human Whole PBMC

Primary Incubation

1:100 for 30 min at 4 degrees Celcius

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

Very good and bright antibody.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Great staining; bright signal

The Bad

None

The Bottom Line

Very good antibody.

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