Human CD16 Antibody

Cancer Center
George Washington University
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BD Biosciences

Product Name:

APC Mouse Anti-Human CD16

Catalog Number:

561304

Our lab works on an antibody with enhanced capacity to mediate ADCC against HIV-infected cells. We set up in vitro assays with purified NK cells and HIV-infected target cells to test the capacity of the antibody to mediate clearance of infected cells by NK cells. Given that CD16 (FcgammaRIIIa) is the primary receptor expressed on NK cells that binds to the anti-HIV antibody, it is crucial for us to detect the expression levels of the receptor in order to interpret the assay results correctly.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Purified CD56+ Cells from human PBMC

Primary Incubation

1:50, 30 min, 4 degree Celcius

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

Antibody works well, and I would recommend its use.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Works great.

The Bad

None

The Bottom Line

Good antibody.

Share your experience with other scientists. Write a Review! »

Join the discussion