Anti-dsRNA Antibody

Microbiology and Immunology
Loyola University Chicago
Postdoc fellow

Overall

Quality of Results

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Company:

Scicons

Product Name:

anti-dsRNA mAb K1

Catalog Number:

10020200

The main goal of my research is to investigate the mechanism by which viruses prevent activation of innate immune response. The goal of this experiment is to determine the presence and localization of viral dsRNA during coronavirus infection. First of all, this company provides the only commercial available anti-dsRNA Abs. This anti-dsRNA antibody (K1) has been used widely as the other clone J2, but it has even a broader application, such as immunoprecipitation and FACS.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse macrophages

Primary Incubation

anti-dsRNA K1 1:500

Blocking Agent

2% BSA

Secondary Incubation

Goat anti-mouse IgG Alexa fluor 568 (1:1000) (Thermofisher, A11004)

Tertiary Incubation

NA

Detection

Cells were imaged by collecting Z-stack images with a Deltavision wide field fluorescent microscope (Applied Precision, GE)

Results Summary

Viral dsRNAs (red) are clearly stained in infected cells as stained with viral protein (green), but not in mock-infected cells.Blue: DAPI stained nucleus

Additional Notes

None

Related Categories

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Summary

The Good

High specificity

The Bad

Not found

The Bottom Line

Reasonable price but good quality!

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