Miltenyi Biotec
autoMACS® Pro Separator
130-092-545
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This review is for the autoMACS Pro, which has been discontinued and replaced by the autoMACS Neo.
Our lab routinely separated different cell populations from human PBMCs (CD8, CD4, CD56...).
Separation of different cell populations from human PBMCs (as well as other organisms)
Human PBMCs
PBMCs are purified from whole blood, counted and incubated with the beads needed to separate (or deplete) a specific cell population. After 30min incubation with beads the cells are resuspended in running buffer and set up in the machine which does the rest of the work.
I always separate the sample in 5 15ml conical tubes (no matter the starting cell number). I get a better yield that way.
The machine works great when it works. We had many problems with it and the Miltenyi reps always came to fix whatever was wrong as long as the machine was still under the service contract/warranty. After that they wanted to charge us up to $8000 for a single visit to come and fix the machine. We eventually stopped using it due to the high maintenance cost. The manual separation is more time consuming but overall slightly faster.
Many technical problems. With frequent use we needed someone to come out and fix small things every few months.
None
Automatic, fast
Breaks a lot
Fast and saves some time that you can spend doing other things. But breaks down a lot so not necessarily worth the money.
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