Revvity
HIV p24 (high sensitivity) AlphaLISA Detection Kit
AL291C
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Our lab uses this kit routinely to to quantify p24 levels in culture supernatant. This is a highly sensitive, fast and easy assay to run.
Quantification of p24 levels
GXR cells (human CEM-derived cell line that is infectable with HIV) co-cultured with human CD8+ T cells
p24 standard and dilutions of your samples are prepared. Samples and standard are incubated with "p24 acceptor beads" for 1h at room temperature. After this incubation "Streptavidin donor beads" are added to the wells and incubated for another 30min at room temperature. After the second incubation the plate is read on an Alpha Elisa plate reader (EnSpire Multilabel Alpha Reader)
Accurate pipets and consistent pipets are essential.
Standard curve consistently comes out perfect. The assay detects even very small levels of p24 in the supernatant.
None
Fast, sensitive, consistent
Need a special plate reader with the Alpha option, Slightly complicated Prism file set up by Revvity rep for data analysis.
Great fast assay for p24 quantification.