Invitrogen Antibodies by Thermo Fisher Scientific
Phospholamban (2D12)
MA3-922
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Browse Similar Products: PLN Western Blot Antibodies
Purpose: visualize monomeric PLN content on PVDF membranes. Methods: 30 ug of soleus homogenate protein were electrophoretically separated using tricine-based SDS PAGE (13% total acrylamide) and then transferred onto PVDF membranes. PLN antibody (1:2000) was used to detect monomeric PLN (~8 kDa). Can also be used to visualize pentameric (25 kDa) if samples are not boiled prior to electrophoresis.
Western Blot
Mouse soleus muscle homogenate
1:2000, 1 hour at room temp
5% milk in TBST
1:2000, goat anti-mouse, 1 hour at room temp
N/A
ECL with Luminata Forte HRP substrate (Millipore)
Monomeric PLN is clearly observed below 10 kDa.
None
Reliably detects monomeric protein
A non-specific band above the pentameric band (25 kDa) can be observed.
Great antibody to use for Western blotting.