Transfer With Ease And Homemade Transfer Buffer

Brock University
Health Sciences
Postdoctoral fellow

Overall

Performance

Ease-of-Optimization

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Company:

BioRad

Product Name:

TransBlot Turbo

Catalog Number:

1704150

Info: Supplier Page 

The Trans-Blot Turbo is a high-performance western blotting transfer system designed to provide rapid transfers with high efficiency - and it does just that! Depending on the percent of gel you are running and the molecular weight of your target protein, you can finish a transfer in as little as 3 min or 7 min. It can also be used as a standard semi dry system, and can be combined just as efficiently with homemade transfer buffer!

Experimental Design and Results Summary

Tips

Stacks can be re-used for up to a month provide that you place them in a sealed tray with transfer buffer. The liners that come with the kits will help ensure that the stacks stay in line with each other.

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Summary

The Good

The speed and efficiency.

The Bad

There is quite a bit of heat at the end of the transfer, and this may worry some researchers; but I wouldn't be too worried about it.

The Bottom Line

Amazing product and much more dynamic and versatile than people think. Don't think that if you purchase this system that you have to be locked down to the protocols listed online or on the product sheet! Enjoy your free time.

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