HLA-DR Expression In Dogs

Vet. pathobiology
University of Missouri
scientist

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Company:

BD Biosciences

Product Name:

FITC-conjugated Mouse Anti-Human HLA-DR

Catalog Number:

556643

Purified canine PMN from blood samples were evaluated for surface HLA-DR expression by using FITC-conjugated Mouse Anti-Human HLA-DR (BD Biosciences). FITC-conjugated Mouse Anti-Human HLA-DR were working efficiently on PMN from dogs.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Blood from dogs

Primary Incubation

1:1000 dioution, 20 minutes on ice

Blocking Agent

FBS in PBS

Secondary Incubation

No need, since FITC conjugated

Tertiary Incubation

No need

Detection

Flow cytometry

Results Summary

FITC-conjugated Mouse Anti-Human HLA-DR (BD Biosciences) were worked efficiently on the canine PMN from blood samples. Results indicate 33±11% of the canine PMN were HLA-DR positive.

Additional Notes

None

Related Categories

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Summary

The Good

Easy to use and time saving.

The Bad

None.

The Bottom Line

Pretty good quality and easy to perform

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