Anti-human CD8 Antibody Review

Cancer Center
George Washington University
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

TONBO Biosciences

Product Name:

APC-Cyanine7 Anti-Human CD8a (RPA-T8)

Catalog Number:

25-0088

Our lab studies the specific killing of HIV-infected cells by CTls and NK cells. Due to complex FACS analysis and the use of multiple colors, we are testing different antibodies in different colors to identify what we can use in our future panels. In this case human T cells were stained with CD3 and the CD8-APC/Cy7 antibody from Tonbo to look at the quality of staining. We obtained good staining for FACS analysis.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human T cells

Primary Incubation

1:100 for 30 min at 4 degrees Celcius

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

We detected good staining of CD8 T cells with this antibody.

Additional Notes

I will use this antibody in teh future

Related Categories

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Summary

The Good

It works

The Bad

None

The Bottom Line

Good antibody.

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