Distinguish Monocytes From Epithelial Cells

Toxicology
University of Kentucky
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Alexa Fluor® 647 anti-human CD11a Antibody

Catalog Number:

301218

One of our experiments involve the mixing of epithelial cells with a monocytic cell line (THP-1) in order to test their response. However, when the cells are harvested, there needs to be a marker that can distinguish the monocytes from epithelial cells. CD11a is a very good candidate.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human THP-1 cells

Primary Incubation

30 minutes (on ice)

Blocking Agent

Human IgG solution (Sigma)

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow Cytometer ~668 nm

Results Summary

The results show clear positive THP-1 cells (red) compared to isotype control (blue).

Additional Notes

None

Related Categories

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Summary

The Good

Easy, easy, easy!

The Bad

None

The Bottom Line

Great and easy way to distinguish monocytes from other cells.

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