Human CD8 Antibody

Cancer Center
George Washington University
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

TONBO Biosciences

Product Name:

violetFluor™ 450 Anti-Human CD8a (RPA-T8)

Catalog Number:

75-0088-T025

We perform in vitro killing assays to detect the effector function of primary CD8 T cells against HIV-infected cells. In this experiment we stained with antibodies against human CD3 and human CD8 (TONBO antibody) to detect CD8 T cells via flow cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMC

Primary Incubation

1:50, 30 min, 4 degree Celcius

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

Antibody worked well to detect human CD8 T cells

Additional Notes

None

Related Categories

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Summary

The Good

Antibody works well and is cost effective

The Bad

None

The Bottom Line

I recommend using this antibody

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