Anti-Human CD4 for Flow Cytometry

Microbiology & Immunology
Albert Einstein College of Medicine
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

TONBO Biosciences

Product Name:

PerCP-Cyanine5.5 Anti-Human CD4 (OKT4)

Catalog Number:

65-0048-T025

We use anti human CD4 antibodies in our HIV research to stain human PBMC. We previously used antibodies from different companies but decided to switch to TONBO due to their lower prices.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

1:100, 30min, 4C

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry PerCP-Cy5.5

Results Summary

The antibody works great for staining human PBMCs. When gating on human CD3+ cells (T cells) I get a population of about 75% CD4 positive cells which is within the expected range.

Additional Notes

None

Related Categories

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Summary

The Good

Great staining with 1:100 dilution of antibody

The Bad

None

The Bottom Line

Great antibody for FACS

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