Anti Human CD8a

Microbiology & Immunology
Albert Einstein College of Medicine
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

TONBO Biosciences

Product Name:

violetFluor™ 450 Anti-Human CD8a (RPA-T8)

Catalog Number:

75-0088-T025

We use anti human CD8a antibody routinely to stain human PBMCs, purified primary CD8+ T cells as well as cell lines to use in flow cytometry experiments. We previously used antibodies from BioLegend but have decided to switch to TONBO due to the lower prices.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

1:50, 30min, 4C

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry, Pacific Blue channel

Results Summary

I used the antibody to stain human PBMCs and got a predictable fraction of CD8 positive cells (18%). The antibody worked well at a reasonable dilution.

Additional Notes

None

Related Categories

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Summary

The Good

Good staining, good price

The Bad

None

The Bottom Line

Good standard antibody. Works well for staining human PBMCs, purified CD8+ T cells and cell lines.

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