Santa Cruz Biotechnology
Survivin Antibody (D-8)
sc-17779
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Browse Similar Products: Survivin Immunofluorescence Antibodies
Survivin is part of the CPC, and this antibody was used in IF experiments to look at components of the CPC in mitosis. Overall the antibody is easy to use and works fairly well. Best if used overnight at 4C. Protocol is below: Cells were fixed in 3.5% paraformaldehyde for 15 minutes at room temperature. 3 minutes in ice-cold methanol. 5 minutes in PBS+0.5% Triton X5 minutes in PBS 10 minutes in PBS+3% BSA Antibody was incubated in PBS+1% BSA and coverslips incubated overnight at 4C2 - 5 minute washes in PBS Secondary antibodies from Jackson Laboratories (anti-rabbit) was incubated in PBS+1% BSA and coverslips 2 - 5 minute washes in PBS Mounted coverslips using ProLong Gold
Immunofluorescence
RPE-1 cells
1:1000 in PBS+1% BSA overnight at 4C
PBS+3%BSA
1:1000 in PBS+1%BSA for 1 hour
None
Florescence imaging was done on a 60X objective
Immunofluorescence images were collected using a 60X objective on a Nikon microscope. Cells were co-stained with DAPI (blue).
It works well
For best signal should be incubated at 4C overnight
Good antibody to look at Survivin. Seems to be specific on Westerns as well (not shown here).