Cell Signaling Technology
Phospho-Akt (Ser473) Antibody
9271
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Since Akt promotes cell survival by inhibiting apoptosis throughits ability to phosphorylate and inactivate several targets, we studied the expression of pAkt during cold storage and rewarming of mouse renal tubual cells.The expression of pAkt was decreased when cells were subjected to cold storage and rewarming.
Western Blot
Mouse renal tubuar cells
1:250 in 2% skimmed milk 4degree overnight
Skimmed milk 5%
1:1000 in 2% skimmed milk 2h at room temperature
N/A
ECL
In mouse reanl tubular cells subjected to cold storage and rewarming the expression of pAkt was significantly decreased compared to control cells.
None
Does not need optimizationlike other phospho antibody.
No bad
Very good antibody to detect the phosphorylated form of Akt at ser 473 position