Reliable High Throughput Surface Plasmon Resonance System

We have used the Reichert SR7500DC for at least 5 years. In that time our application has been study the reconstitution and activity of proteins interacting with liposomes that were immobilized on the SPR surface. We have employed this system to study the binding of BCl-2 family proteins to liposomes and the removal of cholesterol from liposomes. In both cases the SR7500DC has permitted the quantitative study of the kinetics in a way that was not possible without the instrumentation. The reliability and stability of these measurements coupled with the programability for high-throughput assay development has been critical in allowing inhibitor and activator discovery and characterization. These experiments have immediate implications for translational application in the understanding and treatment of atherosclerosis and cancer therapeutics.