BD Biosciences
PE Rat Anti-Mouse CD8a Clone 53-6.7 (RUO)
561095
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The aim of this project is to study tumor cytotoxic T-lymphocytes in a mouse model of colitis-associated colon cancer. In this experiment, we stained for CD8 T-cells in mouse colon cancer tissue sections. This antibody was recommended by my colleague.
Immunofluorescence
Frozen sections of mouse colon cancer tissue
1:200 dilution in blocking buffer; overnight incubation at 4 deg Celsius
1x PBS + 1% BSA + 1% goat serum
No
DAPI was included in the last wash step
Confocal Imaging
CD-8 positive T-cells (red color) were detected in the colon of colitis-associated cancer in mouse. Shown in green color are the epithelial cells.
None
Specific signal
Works well for IF staining