Ep-CAM Antibody For IF Staining

October 04, 2016

Pathology
Stanford University
Postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Biotin anti-mouse CD326 (Ep-CAM) Antibody

Catalog Number:

118203

The aim of this study is to study tumor cell death in colon cancer. In this present experiment, in a mouse model of colitis associated cancer, colonic sections were stained for visualizing epithelial cells.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse colon tissue sample

Primary Incubation

1:200 dilution in blocking buffer; overnight at 4 deg Celsius

Blocking Agent

1x PBS + 1% BSA + 1% goat serum

Secondary Incubation

1:200 dilution; Streptavidin-FITC; 1 hr at r/t

Tertiary Incubation

No

Detection

Confocal Imaging

Results Summary

This antibody clearly stains epithelial cells

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Specific stain

The Bad

No

The Bottom Line

Good antibody for IF staining

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