Thermo Fisher Scientific
CD8 Ab-1 (Clone C8/144B) Mouse Monoclonal Antibody
MS-457-S0
Browse Similar Products: CD8 Immunofluorescence Antibodies
The aim of this project is to study different types of T-cells in human colon cancer. In this particular experiment, human colon cancer tissue microarray is stained with CD8 antibody. I had purchased this antibody with no prior knowledge on whether this performs well for this specific purpose.
Immunofluorescence
Human colon cancer tissue microarray
1:100 dilution in blocking buffer; over night incubation at 4 deg Celsius
1xPBS + 1% BSA + 1% goat serum
1:200 dilution (in 1xPBS + 1% BSA) of Goat-anti mouse Alexa dye 488; 1 hour at r/t0 dilution (in 1xPBS + 1% BSA) of Goat-anti rabbit Alexa dye 594; 1 hour at r/t
DAPI included in the last wash step
Confocal Imaging
CD8 T-cells were clearly detected in the human colon cancer tissue microaarray core biopsy sections.
None
Specific stain
No
It is a good product for IF staining of CD8 positive cells in human tissue sections. One can appreciate the highly specific staining seen in the attached image.