Sigma-Aldrich
Anti-CYP26A1 (481-495) antibody produced in rabbit
C6498-200UL
Browse Similar Products: CYP26A1 Immunofluorescence Antibodies
The aim of this project is to study the role of retinoic acid in the regulation of intestinal immunity. We check whether the expression of retinoic acid metabolizing enzymes changed during disease progression from normal to colitis to cancer. I just tested this product from Sigma.
Immunofluorescence
Human ulcerative colitis tissue specimen
1:1000 dilution in blocking buffer; overnight at 4 deg Celsius
1xPBS + 1% BSA + 5% goat serum
1:200 dilution (in 1xPBS + 1% BSA) of Goat-anti rabbit Alexa dye 594; 1 hour at r/t
DAPI included in the last wash step
Confocal Imaging
In human ulcerative colitis tissue specimens, CYP21A1 expression is elevated compared to normal colon samples
None
Specific stain
No
Highly recommended for IF staining purpose