Invitrogen
Alexa Fluor 555 goat anti-mouse IgG
A21424
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I use immunofluorescence assays to visualize viral infections in cell cultures. I use this antibody to visualize one of my viruses of interest.
Immunofluorescence
Cell culture
Diluted 1/200, 1 hour at 37 degrees celsius
7% normal goat serum
N/A
Fluorescent microscope
This antibody had little background which was very nice. It provided good results in the sense that I could distinguish my infected cells from uninfected cells.
None
Little background and easy to use
Had to use a lower dilution
This antibody gives me good results and I will continue to use it in my experiments.