Nice Product To Isolate Mouse CD8 T Cells

Microbiology and Immunology
Penn State University College of Medicine
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Company:

Miltenyi Biotec Inc.

Product Name:

CD8a (Ly-2) MicroBeads, mouse

Catalog Number:

130-049-401

Mouse CD8a (Ly-2) MicroBeads have been developed for positive selection or depletion of mouse CD8a+ T cells from single-cell suspensions of lymphoid and non-lymphoid tissues or from peripheral blood.

Experimental Design and Results Summary

Applications

Cell Isolation/Separation

Sample

Peripheral blood

Primary Incubation

Cells can be immediately used for downstream applications

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

Cells can be immediately used for downstream applications

Detection

Flow Cytometry

Results Summary

Purified CD8a+ cells have been used for in vitro and in vivo studies on protective immune responses against parasites or allergens and for adoptive transfer into immunodeficient and virus-infected mice.6 Highly pure CD8+ T cells were also isolated from the CNS of MHV-infected mice for evaluation of their chemokine expression pattern. Furthermore, CD8a MicroBeads have been used for the isolation of CD8+CD4+ (DP) thymocytes and the effect of PD-1 expression on their maturation was studied.

Additional Notes

None

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Summary

The Good

High quality

The Bad

Expensive

The Bottom Line

To get good result, you have to choose it.

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