A Good IFA Antibody

Biological Sciences
University of Idaho
Graduate student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Invitrogen

Product Name:

Alexa Fluor 488 Goat anti-rabbit IgG

Catalog Number:

A11034

I use this to visualize viral infections in cell culture and characterize virus replication dynamics. I use this antibody to visualize one of my viruses of interest.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Cell culture

Primary Incubation

Diluted 1/200, 1 hour at 37 degrees celsius

Blocking Agent

7% normal goat serum

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Fluorescent microscope

Results Summary

This antibody may have some high background when using, but it can be optimized to reduce the level of background. However, it is still easy to distinguish my infected cells from uninfected cells.

Additional Notes

None

Image Gallery

Summary

The Good

Easy to optimize and bright results.

The Bad

Little trouble with background

The Bottom Line

This antibody can be optimized to obtain better and more clear results so I would still recommend it.

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