Droplet Digital PCR Efficiency

Durban University of Technology
IWWT
Post doctoral Fellow

Overall

Performance

Ease-of-Optimization

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Company:

Bio-Rad Laboratories

Product Name:

BioRad QX200 Droplet Digital PCR

Catalog Number:

186-3001

I examined the efficacy of droplet digital PCR assay and compared it with qPCR, for quantitative enumeration of bacterial pathogens in environmental samples.

Experimental Design and Results Summary

Tips

There should be minimum time gap between droplet generation and PCR

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Summary

The Good

Easy to handle and highly tolerant to PCR inhibitors

The Bad

Digital droplet PCR showed higher variability and less good precision at the higher concentrations of bacterial pathogens

The Bottom Line

QX200 droplet digital PCR system may provide an opportunity to reduce the inhibitory effects of PCR inhibitors experienced with qPCR, but the methodology need to be further optimized for complex environmental samples.

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