Cell Signaling Technology
Akt Antibody
9272S
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Browse Similar Products: Akt Western Blot Antibodies
I have developed a model of drug resistance to an anti-cancer drug, and performed proteomic analysis to understand the molecular signling pathways that confer resistance. Vials of the antibodies were leftover in the lab from previous use by other investigators. Generally, I prefer Cell Signaling antibodies because they are well-validated and tend to perform well.
Western Blot
Ovarian cancer cell line lysates
1:1000 in 5% milk in TBS-T, O/N at 4 degrees C
5% milk in TBS-T, 1 hr, RT
1:5000 anti-Rb HRP in 5% milk in TBS-T, 30 min, RT
N/A
HRP Chemiluminescence
Very clean blot, only single bands observed.
None
Single band, very clean!
None!
Highly recommend, good antibody.