Bethyl Laboratories Inc
PKD2 antibody
A300-073A
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Protein Kinase D is family of Ser/Thr kinases that regulate multiple biological functions including cell migration, proliferation, apoptosis, transcriptional activation, angiogenesis, and Golgi organization and vesicle trafficking. In the present study, the molecular basis of Protein Kinase D recruitment to the Golgi complex was investigated. I had discovered that class I and Class II ARF proteins and more specifically ARF1 can function as a tethering factor for Protein Kinase D at the Golgi complex. These conclusions were based on protein-protein interaction assays, colocalization experiments, and Golgi functional assays after knockdown down of Protein Kinase D in cells.
Western Blot
HeLa cells; 100 µg of whole cell extract
1:1000 dilution in blocking agent for 16 hr at 4 deg C
5% milk + 0.1% Tween-20 in 1x TBS
Donkey anti-rabbit IgG-HRP conjugated
None
ECL regular (chemiluminescence)
PRKD2 runs at ~110 kDa on a 8% Tris-Glycine gel and the specificity of the band was established by knocking down the transcript with siRNA against PKD2 and a decrease in protein level of PKD2.
Good signal
Good antibody for PKD2 detection by Western Blotting