SIGMA
Jumpstart Taq DNA Polymerase Kit
D4184
JumpStart Taq DNA polymerase is a hot start enzyme, which is in activated by Taq specific antibody. This antibody prevents any non-specific amplification. At 70 degrees, the enzyme activity is restored and non-specific amplifications are highly minimized using this kit.
Cloning / semi-quantitative analysis
DNA
This kit can be used by following the manufacturer's instructions. Briefly, 0.4 microliters of enzyme is used for standard 25 microliter reaction, which contains buffer, dNTPs, magnesium chloride, and appropriate primers of interested genes.
Do not use more volumes of the enzyme
Clear sharp bands are obtained without any non-specific bands
None
The enzyme is very robust and efficient
I did not face any problems
I strongly recommend you this kit to people facing nonspecific amplification