Novus Biologicals
TGN46 Antibody
NBP1-49643
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Protein Kinase D is family of Ser/Thr kinases that regulate multiple biological functions including cell migration, proliferation, apoptosis, transcriptional activation, angiogenesis, and Golgi organization and vesicle trafficking. In the present study, the molecular basis of Protein Kinase D recruitment to the Golgi complex was investigated. TGN46 antibody was used as a marker for colocalization experiments. This antibody was recommended by another researcher working on Golgi complex structure.
Immunofluorescence
HeLa cells
1:1000 dilution; 1 hr at room temp
1% BSA+0.05% Saponin in 1xPBS
Goat anti-rabbit Alexa Fluor 488; 1:1000 dilution; 1 hr at room temperature
None
Confocal Microscopy
TGN46 antibody from Novus Biologicals is a good marker for the staining the trans-Golgi compartment in HeLa cells
Specificity of the signal
A good antibody for trans-Golgi network staining